Anti-Human BAFF Recombinant Antibody (TAB-216)
CAT#: TAB-216
Recombinant monoclonal antibody to BAFF. It is an anti-B-cell activating factor (BAFF) human monoclonal antibody designed for the treatment of autoimmune diseases and B cell malignancies.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30526/1926_A6_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, Focal adhesion sites & Vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30526/1702_E2_13_cr5db6ea10de8f3_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to Vesicles & Focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30526/1708_E6_34_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_A_8_8.jpg
Normal Tissue
Figure 9 Tonsil
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Squamous epithelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9188/24697_A_5_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (U-937)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102524-TNFSF13B
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❯
SDS-PAGE
Figure 1 Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in SDS-PAGE
Lane 1: Reduced mAb
Lane 2: Non-reduced mAb
SEC-HPLC
Figure 2 Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in SEC-HPLC
The purity of TAB-216-ADCP was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in WB
Western blot analysis of TAB-216-ADCP was performed by loading recombinant Human BAFF with his-tag.
TAB-216-ADCP incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-human IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
ELISA
Figure 4 Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in ELISA
ELISA analysis of TAB-216-ADCP was performed by coating with recombinant Human BAFF with his-tag.
The secondary antibody: HRP-Goat anti-human IgG
DB
Figure 5 Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in DB
Anti-Human BAFF Recombinant Antibody, ADCP enhanced (TAB-216-ADCP) in DB
Dot Blot analysis of TAB-216-ADCP was performed by coating with recombinant Human BAFF with his-tag.
The secondary antibody: HRP-Goat anti-human IgG
SDS-PAGE
Figure 6 Anti-Human BAFF Recombinant Antibody in SDS-PAGE
SDS-PAGE analysis of TAB-216 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 7 Anti-Human BAFF Recombinant Antibody in SEC-HPLC
The purity of TAB-216 was greater than 95% as determined by SEC-HPLC.
WB
Figure 8 Anti-Human BAFF Recombinant Antibody in WB
Western blot analysis of TAB-216 was performed by loading human BAFF protein, His tag.
TAB-216 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L).
Lane 1: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 9 Anti-Human BAFF Recombinant Antibody in ELISA
ELISA analysis of TAB-216 was performed by coating with human BAFF protein (His tag). Then blocked with BSA and incubated with TAB-216. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
❮
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG4 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- FuncS, IF, Neut, ELISA, FC, IP, IHC
- MW
- 146.25 kDa
- Related Disease
- Connective tissue disease
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Storage
- 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.
Applications
- Application Notes
- The TNFSF13B antibody has been reported in applications of Activ, Neut, PD.
Target
- Alternative Names
- Tabalumab;1143503-67-6;LY2127399;LY 2127399;TNFSF13B;tumor necrosis factor (ligand) superfamily, member 13b;TNFSF20;tumor necrosis factor ligand superfamily member 13B;BAFF;BLYS;CD257;TALL 1;TALL1;THANK;delta BAFF;Delta4 BAFF;B-lymphocyte stimulator;B-cel
- Gene ID
- 10673
- UniProt ID
- Q9Y275
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(Creative Biolabs Cat# TAB-216, RRID: AB_3111877)
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Biosimilar Overview
Please refer to Tabalumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tabalumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1226z | Mouse Anti-TNFSF13B Recombinant Antibody (clone 25D8) | WB, IP, IF, ELISA | Mouse IgM, κ |
| HPAB-0121-FY | Mouse Anti-TNFSF13B Recombinant Antibody (HPAB-0121-FY) | ELISA | Mouse IgG |
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| FAMAB-0324JF | Mouse Anti-TNFSF13B Recombinant Antibody (clone ABL-1) | WB, ELISA, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-024 | Recombinant Anti-Human TNFSF13B VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
| TAB-0055CL-VHH | Anti-Human TNFSF13B VHH Single Domain Antibody (TAB-0055CL-VHH) | ELISA, FuncS, Inhib, BLI | Single domain antibody |
| HPAB-0235-YJ-VHH | Recombinant Llama Anti-TNFSF13B Single Domain Antibody (HPAB-0235-YJ-VHH) | ELISA, Inhib | Llama VHH |
| HPAB-0236-YJ-VHH | Recombinant Llama Anti-TNFSF13B Single Domain Antibody (HPAB-0236-YJ-VHH) | ELISA, Inhib | Llama VHH |
| HPAB-0237-YJ-VHH | Recombinant Llama Anti-TNFSF13B Single Domain Antibody (HPAB-0237-YJ-VHH) | ELISA, Inhib | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1158CL | Anti-Human TNFSF13B Recombinant Antibody (6C10) | ELISA, Inhib | Fully human antibody |
| TAB-1159CL | Anti-Human TNFSF13B Recombinant Antibody (10C11) | ELISA, Inhib | Fully human antibody |
| TAB-1160CL | Anti-Human TNFSF13B Recombinant Antibody (6A11) | ELISA | Fully human antibody |
| TAB-1161CL | Anti-Human TNFSF13B Recombinant Antibody (5B7) | ELISA, Inhib | Fully human antibody |
| TAB-1162CL | Anti-Human TNFSF13B Recombinant Antibody (1B7) | ELISA, Inhib | Fully human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1179CL | Human Anti-TNFSF13B Recombinant Antibody (TAB-1179CL) | Neut | Chimeric (Mouse/Human) antibody |
| TAB-1180CL | Human Anti-TNFSF13B Recombinant Antibody (TAB-1180CL) | Neut | Chimeric (Mouse/Human) antibody |
| TAB-1179CL-F(E) | Human Anti-TNFSF13B Recombinant Antibody; Fab Fragment (TAB-1179CL-F(E)) | Neut | Chimeric (Mouse/Human) Fab |
| TAB-1180CL-F(E) | Human Anti-TNFSF13B Recombinant Antibody; Fab Fragment (TAB-1180CL-F(E)) | Neut | Chimeric (Mouse/Human) Fab |
| TAB-1182CL-F(E) | Anti-Human TNFSF13B Recombinant Antibody Fab Fragment (H1104-30-CA) | ELISA, Inhib | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1177CL-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1177CL-S(P)) | Neut | Mouse scFv |
| TAB-1178CL-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1178CL-S(P)) | Neut | Mouse scFv |
| TAB-1181CL-S(P) | Anti-Human TNFSF13B Recombinant Antibody scFv Fragment (H1104-30) | ELISA, Inhib | |
| TAB-1183CL-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1183CL-S(P)) | ELISA, Inhib | Mouse scFv |
| TAB-1184CL-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1184CL-S(P)) | ELISA, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1194CL-S(P) | Human Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1194CL-S(P)) | ELISA | Human scFv |
| TAB-1195CL-S(P) | Human Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1195CL-S(P)) | ELISA | Human scFv |
| TAB-1196CL-S(P) | Human Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1196CL-S(P)) | ELISA | Human scFv |
| TAB-1197CL-S(P) | Human Anti-TNFSF13B Recombinant Antibody; scFv Fragment (TAB-1197CL-S(P)) | ELISA | Human scFv |
| TAB-1194CL-F(E) | Human Anti-TNFSF13B Recombinant Antibody; Fab Fragment (TAB-1194CL-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2184CQ | Rat Anti-TNFSF13B Recombinant Antibody (clone 8F10) | Neut, FC, IHC, IHC-Fr | Rat IgG2a |
| NEUT-2185CQ | Mouse Anti-TNFSF13B Recombinant Antibody (clone CBL701) | Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3973 | Rabbit Anti-TNFSF13B Recombinant Antibody (clone SI33DS) | WB, FC, IP | Rabbit IgG |
| MOR-4470 | Rabbit Anti-TNFSF13B Recombinant Antibody (clone SI564DS) | FC | Rabbit IgG |
| MOR-4471 | Rabbit Anti-TNFSF13B Recombinant Antibody (clone SI565DS) | ELISA | Rabbit IgG |
| MOR-4537 | Rabbit Anti-TNFSF13B Recombinant Antibody (clone TH45DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0235-YC-S(P) | Human Anti-TNFSF13B Recombinant Antibody; scFv Fragment (HPAB-0235-YC-S(P)) | ELISA, Neut | Human scFv |
| HPAB-0121-FY-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody; scFv Fragment (HPAB-0121-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0730LY-S(P) | Human Anti-TNFSF13B Recombinant Antibody (clone CBL016); scfv Fragment | ELISA | Human scfv |
| HPAB-1255WJ-S(P) | Human Anti-B lymphocyte stimulator protein Recombinant Antibody; scFv Fragment (HPAB-1255WJ-S(P)) | ELISA, WB | Human scFv |
| FAMAB-0324JF-S(P) | Mouse Anti-TNFSF13B Recombinant Antibody (clone ABL-1); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0730LY-F(E) | Human Anti-TNFSF13B Recombinant Antibody (clone CBL016); Fab Fragment | ELISA | Human Fab |
| HPAB-N0286-YC-F(E) | Human Anti-TNFSF13B Recombinant Antibody (clone h8E7D9C7F5-1); Fab Fragment | ELISA, FC, Inhib | Human Fab |
| HPAB-1255WJ-F(E) | Human Anti-B lymphocyte stimulator protein Recombinant Antibody; Fab Fragment (HPAB-1255WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-1256WJ-F(E) | Human Anti-B lymphocyte stimulator protein Recombinant Antibody; Fab Fragment (HPAB-1256WJ-F(E)) | ELISA, WB | Human Fab |
| FAMAB-0324JF-F(E) | Mouse Anti-TNFSF13B Recombinant Antibody (clone ABL-1); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-030 | Afuco™ Anti-TNFSF13B ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-030) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-216 | Afuco™ Anti-TNFSF13B ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-216) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1350 | AbPlus™ Anti-TNFSF13B Magnetic Beads (VS-0724-YC1350) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY47 | Human Anti-TNFSF13B (clone 10B1) scFv-Fc Chimera | ELISA, Neut | Human IgG1, scFv-Fc |
| VS-0125-FY48 | Human Anti-TNFSF13B (clone 1B5) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| VS-0425-FY68 | Human Anti-TNFSF13B scFv-Fc Chimera (VS-0425-FY68) | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY226 | CytoStream™ Mouse Anti-TNFSF13B Recombinant Antibody (clone H32-411) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC601 | Recombinant Anti-TNFSF13B Vesicular Antibody, EV Displayed (VS-0425-YC601) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7394 | Anti-TNFSF13B Immunohistochemistry Kit | IHC |
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